Validating rnai targets

However, significant barriers to successful si RNA therapies remain, the most significant of which is RNAs have a well-defined structure: a short (usually 20 to 24-bp) double-stranded RNA (ds RNA) with phosphorylated 5' ends and hydroxylated 3' ends with two overhanging nucleotides.



The 5’ fragment is degraded from its 3’ end by exosome while the 3’ fragment is degraded from its 5’ end by 5' -3' exoribonuclease 1(XRN1).The gene knockdown efficiency can also be improved by using cell squeezing.


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